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It has been demonstrated that neostigmine augments clindamycin-induced neuromuscular block ; however, it remains unclear whether neostigmine enhances the neuromuscular block that is caused by a combination of rocuronium and clindamycin.

Becker and Miller have shown that the combination of clindamycin and d-tubocurarine or pancuronium synergistically depresses twitch tension.

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However, there are no reports investigating the effect of clindamycin on rocuronium-induced muscle relaxation or the interaction between clindamycin and rocuronium on neuromuscular block.

The Loewe additivity model has been established as the universal reference model for classifying drug interactions.

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When the interaction index as per the Berenbaum method is equal to, less than or greater than unity, the combination dose is labelled as additive, synergistic or antagonistic, respectively. The intent of this study was to determine whether neostigmine potentiates the muscle relaxation that is induced by combinations of rocuronium and clindamycin and to estimate whether both clindamycin and rocuronium have synergistic actions on neuromuscular block.

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The phrenic nerve and diaphragm were excised together and the left diaphragm with the middle diaphragmatic ligament was separated from the rest of the diaphragm.

Left phrenic nerve-hemidiaphragm preparations were mounted in a 20-ml organ bath that had been filled with Krebs solution 118 mM NaCl, 5 mM KCl.

The pH of the bath solution was maintained at 7. Spent Krebs solution was exchanged with fresh solution 10 min after the preparation was mounted.

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The phrenic nerve, connected to an electrode, was stimulated with supramaximal square wave impulses of 0.

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